Journal:

Article Title: Suppression of Gamma Interferon Transcription and Production by Nematode Excretory-Secretory Antigen during Polyclonal Stimulation of Rat Lymph Node T Cells

doi:

Figure Lengend Snippet: Effect of ES antigen on cytokine production by CD4+ and CD8+ T cells separated from pooled naive rat MLNs. Purified CD4+ and CD8+ T cells (106/ml) were cultured with or without ES antigen under stimulation with anti-CD3/CD28. After 72 h, supernatants were harvested and IFN-γ and IL-4 levels were determined by ELISA. The numbers of viable cells were evaluated by MTS assay (C). The data are the means ± standard deviations of triplicate cultures. The results are representative of three independent experiments. ND, not detectable.

Article Snippet: In brief, MLN cells were incubated for 30 min on ice with a mixture of the following mouse monoclonal antibodies: anti-rat CD45RA, specific for B cells (OX-33; Serotec, Oxford, United Kingdom), and anti-rat CD8 (OX-8; Serotec) for CD4 + T-cell selection or anti-rat CD45RA and anti-rat CD4 (W3/25; Serotec) for CD8 + T-cell selection.

Techniques: Purification, Cell Culture, Enzyme-linked Immunosorbent Assay, MTS Assay

Journal:

Article Title: Influence of Glycosylation on the Efficacy of an Env-Based Vaccine against Simian Immunodeficiency Virus SIVmac239 in a Macaque AIDS Model

doi: 10.1128/JVI.79.16.10386-10396.2005

Figure Lengend Snippet: Env-specific CD4+ T-cell and CD8+ T-cell responses in 12 macaques. A: Env-specific CD8+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups. B: Env-specific CD4+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups. ELISPOT results are colored as follows: Δ5G SU-specific T cells (red), wt-SU-specific T cells (green), and TM-specific T cells (yellow). Arrows with a dotted line, arrows with broken line, and arrows with a solid line indicate the time of the third DNA vaccination at 8 weeks p.p., the time of the vaccine boost at 21 weeks p.p., and the time of SIVmac239 challenge at 28 weeks p.p., respectively. C: Comparison of SU-specific CD8+ T cells and CD4+ T cells in PBMCs among the wt-Env vaccine group, the Δ5G Env vaccine group, and the vector control group at 26 weeks p.p. and 4, 7, and 12 days p.i. The numbers of SFC responding to SIVmac239 SU were used to compare the effects of the two vaccines. w, weeks; d, days.

Article Snippet: PBMCs were subjected to the depletion of CD4 + cells with magnet beads coated with anti-human CD4 Ab (Dynal ASA, Oslo, Norway) or subjected to the depletion of CD8 + cells with magnet beads coated with anti-human CD8 Ab (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Enzyme-linked Immunospot, Comparison, Plasmid Preparation, Vaccines

Journal:

Article Title: Influence of Glycosylation on the Efficacy of an Env-Based Vaccine against Simian Immunodeficiency Virus SIVmac239 in a Macaque AIDS Model

doi: 10.1128/JVI.79.16.10386-10396.2005

Figure Lengend Snippet: SIV-specific CD8+ T-cell and CD4+ T-cell responses in 12 animals. A: SIV viral-protein-specific CD8+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups: vector controls, wt-Env vaccine group, and Δ5G Env vaccines. B: SIV viral-protein-specific CD4+ T cells in PBMCs were measured by ELISPOT assay for IFN-γ in three groups. ELISPOT results of individual SIV proteins are colored as follows: Gag (red), Nef (green), Tat/Rev (blue), Vif/Vpr/Vpx (yellow), and Pol (pink). C: Comparison of cumulated CD8+ T cells or CD4+ T cells specific to the viral proteins Gag, Pol, Nef, Tat/Rev, and Vif/Vpr/VpX between SIV infection-controlled and uncontrolled animals. w, weeks; d, days.

Article Snippet: PBMCs were subjected to the depletion of CD4 + cells with magnet beads coated with anti-human CD4 Ab (Dynal ASA, Oslo, Norway) or subjected to the depletion of CD8 + cells with magnet beads coated with anti-human CD8 Ab (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Enzyme-linked Immunospot, Plasmid Preparation, Vaccines, Comparison, Infection

Journal: Infection and Immunity

Article Title: Local Host Response to Chlamydial Urethral Infection in Male Guinea Pigs ▿

doi: 10.1128/IAI.01339-09

Figure Lengend Snippet: Kinetics of the cellular response in the urethra following primary and challenge infections as assessed by flow cytometry. Each data point represents the mean and standard deviation for 5 animals. The number of CD8 cells on day 14 was significantly greater than the number of CD4 cells (P = 0.008). The number of B cells following challenge infection was significantly greater than the numbers of CD4 and CD8 T cells on each of the days following challenge (P, <0.01 to 0.001).

Article Snippet: R-phycoerythrin (RPE)-labeled mouse anti-guinea pig CD4 (CT7), fluorescein isothiocyanate (FITC)-labeled mouse anti-guinea pig CD8 (CT6), mouse anti-guinea pig CD45 (IH-1), mouse anti-B-cell subset (MsGp10), and an allophycocyanin (APC) conjugation kit were purchased from AbD Serotec (Oxford, United Kingdom).

Techniques: Flow Cytometry, Standard Deviation, Infection